| Registro completo |
| Provedor de dados: |
Acta biol.Colomb.
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| País: |
Colombia
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| Título: |
ANTIFUNGAL ACTIVITY OF NEEM (Azadirachta indica: MELIACEAE) EXTRACTS AGAINST DERMATOPHYTES
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| Autores: |
OSPINA SALAZAR,Daniel Iván
HOYOS SÁNCHEZ,Rodrigo Alberto
OROZCO SÁNCHEZ,Fernando
ARANGO ARTEAGA,Myrtha
GÓMEZ LONDOÑO,Luisa Fernanda
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| Data: |
2015-09-01
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| Ano: |
2015
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| Palavras-chave: |
HPLC
Neem
Microbial sensitivity tests
Minimum inhibitory concentration
Terpenoids
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| Resumo: |
In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal inhibitory concentrations (MIC) between 50 μg/mL and 200 μg/mL for leaves extract, and between 625 μg/mL and 2500 μg/mL for seed oil extract. The MIC of positive control (Terbinafine) ranged between 0.0019 μg/mL and 0.0313 μg/mL. Both neem leaves and seed oil methanol extracts exhibited different chromatographic profiles by HPLC, which could explain the differences observed in their antifungal activity. This analysis revealed the possible presence of terpenoids in both extracts, which are known to have biological activity. The results of this research are a new report on the therapeutic potential of neem to the control of dermatophytosis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017
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| Editor: |
Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
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| Relação: |
10.15446/abc.v20n3.45225
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| Formato: |
text/html
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| Fonte: |
Acta Biológica Colombiana v.20 n.3 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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